M-SRG

品系全名

SD-Rag2em1SmocIl2rgem1Smoc

目錄號(hào)

NR-KO-210360

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名

基因曾用名

Rag-2;gc; p64; [g]c; CD132; gamma?

NCBI ID

RGD ID

Ensembl ID

Pubmed

品系描述

利用CRISPR基因編輯技術(shù)，敲除Rag2-KO(SD)大鼠IL2rg基因的exon 2-7，建立Rag2和Il2rg基因敲除大鼠模型。

驗(yàn)證數(shù)據(jù)

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Fig1. Complete deletion of T, B and NK cells of M-SRG rats. Spleen and peripheral blood cells from SD and M-SRG rats were collected to analyze their compositions of T, B and NK cells by FACS.

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Fig2. Subcutaneous xenograft tumor growth of HT29 cells in M-SRG rats.? Human colorectal adenocarcinoma cell line HT-29 (2x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG rats (n=6). (A)Tumor volume. (B) Body weight change.

image.pngFig3. Subcutaneous xenograft tumor growth of PC-3 cells in M-SRG rats. Human prostatic adenocarcinoma cell line PC-3 (2x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG rats (n=6). (A)Tumor volume. (B) Body weight change.

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Fig4. Subcutaneous xenograft tumor growth of LNCaP clone FGC cells in M-SRG rats. Human metastatic prostate carcinoma cell line LNCaP clone FGC (2x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG rats (n=6). (A)Tumor volume. (B) Body weight change.?

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Fig5. Subcutaneous xenograft tumor growth of A2780 cells in M-SRG rats.? Human ovarian cancer cell line HT-29 (1x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG rats (n=6). (A)Tumor volume. (B) Body weight change.?

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Fig6. Subcutaneous xenograft tumor growth of MV4-11 cells in M-SRG?rats. Human monocytic leukemia cell line MV4-11 (1x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG?rats (n=6). (A)Tumor volume. (B) Body weight change.?

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Fig7. Subcutaneous xenograft tumor growth of OCI-AML-3 cells in M-SRG rats.? Human acute myeloid leukemia cell line OCI-AML-3 (1x107) were mixed with Matrigel and inoculated subcutaneously into M-SRG rats (n=6). (A) Tumor volume. (B) Body weight change.image.png

Fig8. Blood Routine Tests in M-SRG rats.??

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Fig9. Blood biochemistry in M-SRG rats.?

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