小鼠的腎臟被切除5/6后，全身的血液濾過功能將由殘余的腎單位承擔(dān)。劇烈的血流動力學(xué)改變會直接導(dǎo)致腎小球高濾過，進(jìn)而導(dǎo)致腎小球硬化和殘余腎單位的破壞，出現(xiàn)以腎小球肥大、硬化等為主要特點(diǎn)的慢性腎衰竭的腎間質(zhì)纖維化病變。
為保證小鼠存活率，常采取兩步法對小鼠腎臟進(jìn)行切除：
1、適應(yīng)性喂養(yǎng)1周后，麻醉小鼠，并切除小鼠左腎2/3；
2、一期手術(shù)7天后，切除整個(gè)右腎。
Fig.1 Body weight of?5/6 nephrectomy induced CKD model in C57BL/6.
Fig.2 5/6 nephrectomy induced CKD model in C57BL/6. (A) serum UREA; (B) serum CRE.
Fig.3 5/6 nephrectomy induced CKD model in C57BL/6. (A) pathological score; (B) typical pathology image of H&E stain; (C) fibrosis score; (D) typical pathology image of Masson stain.
Fig.4 5/6 nephrectomy induced CKD model in C57BL/6. (A) relative expression of α-SMA mRNA in kidney; (B) relative expression of Col1a1 mRNA in kidney; (C) relative expression of TGF-β mRNA in kidney.
Fig.5 5/6 nephrectomy induced CKD model in C57BL/6. (A) RBC; (B) HGB; (C) HCT%.
Fig.1 Clinical symptoms of CKD mouse model. (A) body weight (B) survival percentage. ***P<0.001 vs Adenine.
Fig.2 Serum biochemical analysis and gross anatomy analysis of CKD mouse model.?(A) serum BUN; (B) serum CRE; (C) left kidney index: left kidney weight / body weight; (D) right kidney index: right kidney weight / body weight . *P<0.05, **P<0.01, ***P<0.001?vs Adenine.
Fig.3 Kidney H&E pathology?of CKD?mouse model. (A) typical pathology image; (B) histological score; (C) MCC950 80mpk can relieve glomerulosclerosis induced by adenine.?*P<0.05, **P<0.01, ***P<0.001?vs Adenine.
