Abcb4-KO(FVB)

品系全名

FVB-Abcb4em1Smoc

目錄號

NM-KO-200584

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
Abcb4

基因曾用名

Mdr2; Pgy2; Pgy-2; mdr-2

染色體位置

5

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

ABCB4

品系描述

敲除Abcb4基因exon 3-4，建立Abcb4基因敲除小鼠模型。
應(yīng)用領(lǐng)域：膽汁等疾病相關(guān)研究

疾病預(yù)測

膽囊炎
Cholecystitis

近似模型的表型

參考文獻(xiàn)

Lammert F, Wang DQ, Hillebrandt S, Geier A, Fickert P, Trauner M, Matern S, Paigen B, Carey MC, Spontaneous cholecysto- and hepatolithiasis in Mdr2-/- mice: a model for low phospholipid-associated cholelithiasis. Hepatology. 2004 Jan;39(1):117-28

膽汁淤積
Cholestasis

近似模型的表型

參考文獻(xiàn)

Hochrath K, Ehnert S, Ackert-Bicknell CL, Lau Y, Schmid A, Krawczyk M, Hengstler JG, Dunn J, Hiththetiya K, Rathkolb B, Micklich K, Hans W, Fuchs H, Gailus-Durner V, Wolf E, de Angelis MH, Dooley S, Paigen B, Wildemann B, Lammert F, Nussler AK, Modeling hepatic osteodystrophy in Abcb4 deficient mice. Bone. 2013 Aug;55(2):501-11

肝內(nèi)膽汁淤積
Intrahepatic Cholestasis

近似模型的表型

參考文獻(xiàn)

Li Z, Agellon LB, Vance DE, The role of phosphatidylethanolamine methyltransferase in a mouse model of intrahepatic cholestasis. Biochim Biophys Acta. 2011 Apr;1811(4):278-83

肝細(xì)胞癌
Hepatocellular Carcinoma

近似模型的表型

參考文獻(xiàn)

Mauad TH, van Nieuwkerk CM, Dingemans KP, Smit JJ, Schinkel AH, Notenboom RG, van den Bergh Weerman MA, Verkruisen RP, Groen AK, Oude Elferink RP, van der Valk MA, Borst P, Offerhaus GJ, Mice with homozygous disruption of the mdr2 P-glycoprotein gene. A novel animal model for studies of nonsuppurative inflammatory cholangitis and hepatocarcinogenesis. Am J Pathol. 1994 Nov;145(5):1237-45

原發(fā)性硬化性膽管炎
Primary Sclerosing Cholangitis

近似模型的表型

參考文獻(xiàn)

Strack I, Schulte S, Varnholt H, Schievenbusch S, Tox U, Wendland K, Steffen HM, Drebber U, Dienes HP, Odenthal M, beta-Adrenoceptor blockade in sclerosing cholangitis of Mdr2 knockout mice: antifibrotic effects in a model of nonsinusoidal fibrosis. Lab Invest. 2011 Feb;91(2):252-61

驗(yàn)證數(shù)據(jù)

image.png

Fig1. Serum biochemistry of Abcb4-KO mice (n=3 female and 3 male).

image.png

Fig2. Significant bile duct hyperplasia and interstitial focal cell infiltration were observed in the liver of Abcb4-KO mice.

(A) Liver were collected from FVB wild-type (+/+) and Abcb4-KO mice, and analyzed by HE staining. (B) Pathological analysis of the liver in Abcb4-KO mice.

image.png

Fig3. Collagen fiber hyperplasia were observed in the liver of Abcb4-KO mice.

(A) Liver were collected from FVB wild-type (+/+) and Abcb4-KO mice, and analyzed by Masson staining. (B) Quantification of the Collagen volume fraction (CVF) .

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