hMAPT

品系全名

C57BL/6Smoc-Mapttm1(hMAPT)Smoc

目錄號

NM-HU-200116

品系狀態(tài)

活體

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基因信息

基因名
Mapt

基因曾用名

Tau; Mtapt; AI413597; AW045860

染色體位置

11

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

MAPT

品系描述

通過同源重組，將小鼠基因Mapt進行人源化修飾。與此相似的品系還有hMAPT(2)(NM-HU-200257),詳細信息請咨詢技術顧問。
應用領域：老年癡呆模型

驗證數(shù)據

image.png

Fig.1 Detection of MAPT expression in cerebellum by RT-PCR.?

Wild type: only one band at 200 bp with primers F1/R1 (mMapt);?

Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 3. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 4. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

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