hMAPT

品系全名

C57BL/6Smoc-Mapttm1(hMAPT)Smoc

目錄號(hào)

NM-HU-200116

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Mapt

基因曾用名

Tau; Mtapt; AI413597; AW045860

染色體位置

11

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類(lèi)同源基因

MAPT

品系描述

通過(guò)同源重組，將小鼠基因Mapt進(jìn)行人源化修飾。與此相似的品系還有hMAPT(2)(NM-HU-200257),詳細(xì)信息請(qǐng)咨詢(xún)技術(shù)顧問(wèn)。
應(yīng)用領(lǐng)域：老年癡呆模型

驗(yàn)證數(shù)據(jù)

image.png

Fig.1 Detection of MAPT expression in cerebellum by RT-PCR.?

Wild type: only one band at 200 bp with primers F1/R1 (mMapt);?

Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 3. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 4. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF.?

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody.?

Abbr. HO/HO, homozygous; +/+, wild type.?

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無(wú)Tamoxifen誘導(dǎo)的情況下，在細(xì)胞質(zhì)內(nèi)處于無(wú)活性狀態(tài)；當(dāng)Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類(lèi)似物）與ERT結(jié)合，可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問(wèn)】基因工程小鼠的命名規(guī)則

常見(jiàn)的基因工程小鼠可以分為兩種命名方式，包括基因定點(diǎn)修飾的小鼠命名，比如：敲除、敲入、點(diǎn)突變等等，和隨機(jī)轉(zhuǎn)基因的小鼠命名。

查看