Panc02-OVA-Luc
品系基本信息
驗證數(shù)據(jù)
Ⅰ.In vitro?Expression of Panc02-OVA-Luc cell line
The protein expression of OVA in the wild-type Panc02 and Panc02-OVA-Luc cells were measured by Western Blot.
Figure?1. OVA expression in wild-type Panc02 and Panc02-OVA-Luc cell lines
Luciferase activity?expression on the Panc02-OVA-Luc?cell?was measured by microplate reader.
Figure 2. Luciferase activity assay of Panc02-OVA-Luc cell line
Ⅱ.Characterization of in vivo?growth kinetics
To verify whether there is the?difference in tumor formation between the Panc02 -OVA-Luc?cell line and the wild-type Panc02?cell line, both cells?were subcutaneously inoculated into C57BL/6 mice.
Figure?3. Tumor growth curve of Panc02 and Panc02-OVA-Luc syngeneic model
Figure?4. Body weight change of Panc02 and Panc02-OVA-Luc syngeneic model
Ⅲ.Expression of OVA protein in tumors
Figure?5. The expression of OVA in tumors by Western Blot
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