Fig1.?Expression of PD-L1 in the spleen lymphocytes collected from homozygous humanized PD-L1 mice and wild-type mice is detected by FACS.?The results showed that the expression of human PD-L1 can be detected in both T cells and B cells collected from the spleen of homozygous humanized PD-L1 mice. (Completed in collaboration with CrownBio)

Fig2. Immunotype in blood , spleen and bone marrow in hPD-L1 mice.??

Fig3. Immunotype in blood in hPD-L1 mice.?

Fig4. Immunotype in spleen in hPD-L1 mice.?

Fig5. Immunotype in bone marrow in hPD-L1 mice.?

Fig6.?In vivo?validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized PD-L1 mice.?Homozygous humanized PD-L1 mice were inoculated with MC38 colon cancer cells (expressing human PDL1 rather than murine PD-L1). After the tumors grew to 100 mm3, the animals were randomly assigned into a control group and a treatment group (n=5). The results showed: The antibodies targeting human PD-L1 were associated with a very significant anti-tumor effect (TGI: tumor growth inhibition, p < 0.001), demonstrating that the humanized PD-L1 mice are a good in vivo model for validating the efficacy of antibodies targeting human PD-L1.

Distinct contribution of PD-L1 suppression by spatial expression of PD-L1 on tumor and non-tumor cells
來(lái)源雜志：Cellular & Molecular Immunology