hPTPN1

品系全名

C57BL/6Smoc-Ptpn1tm1(hPTPN1)Smoc

目錄號(hào)

NM-HU-225059

品系狀態(tài)

胚胎凍存

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基因信息

基因名
Ptpn1

基因曾用名

PTP1B; PTP-1B; PTP-HA2

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

PTPN1

品系描述

利用同源重組，將小鼠Ptpn1基因進(jìn)行人源化修飾。

驗(yàn)證數(shù)據(jù)

image.png

Fig.1 Detection of PTPN1 expression in spleen by RT-PCR.?

Wild type: only one band at 214 bp with primers F1/R1(mPtpn1);

Homozygous: only one band at 206 bp with primers F2/R2(hPTPN1).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB.?

Abbr. M, marker; WT, wild type; HO, homozygous.

Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.

image.png

Fig.3 Detection of human PTPN1 expression in hPTPN1 knockin mice by WB.?

Abbr. M, marker; WT, wild type; HO, homozygous; PC, positive control, Hela cells.

Note. The anti-human PTPN1 antibody cross-reacted with mouse PTPN1 and humanized PTPN1. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.

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