hIL13(BALB/c)

品系全名

BALB/cAnSmoc-Il13em2(hIL13)Smoc

目錄號

NM-HU-220019

品系狀態(tài)

胚胎凍存

導出PDF

基因信息

基因名
IL13

品系描述

通過同源重組,將小鼠Il13基因進行人源化修飾。

驗證數(shù)據(jù)

image.png

Fig1. Detection of IL-13 expression in thymus by RT-PCR.?Wild type: only one band at 260 bp with primers F1/R1(mIl-13);Heterozygous: one band at 260 bp with primers F1/R1(mIl-13) and one band at 334 bp with primers F2/R2(hIL-13); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of hIL13 expression in cell culture supernatant by ELISA.?Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. Splenocytes from BALB/c and homozygous hIL13 mice were stimulated with ConA in vitro.

image.png

Fig3. Detection of mIL13 expression in cell culture supernatant by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. Splenocytes from BALB/c and homozygous hIL13 mice were stimulated with ConA in vitro.


你也可能感興趣

Tamoxifen誘導Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導的情況下,在細胞質內(nèi)處于無活性狀態(tài);當Tamoxifen誘導后,Tamoxifen的代謝產(chǎn)物4-OHT(雌激素類似物)與ERT結合,可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式,包括基因定點修飾的小鼠命名,比如:敲除、敲入、點突變等等,和隨機轉基因的小鼠命名。

查看