hAPOE4

品系全名

C57BL/6Smoc-Apoetm2(hAPOE4)Smoc

目錄號

NM-HU-190002

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Apoe

基因曾用名

Apo-E; AI255918

染色體位置

7

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

APOE

品系描述

通過同源重組，將小鼠Apoe基因進行人源化修飾。
應(yīng)用領(lǐng)域：心腦血管疾病

驗證數(shù)據(jù)

Apoe4-2.png

Fig1. Genotyping of WT 、KO &? Humanized mice by PCR.

Primer P1/2 Wild type = 342 bp

Primer P3/4 mutant = 332 bp

M：100bp Plus DNA ladder.

image.png

Fig2. Detection of human APOE4 expression in Cerebellum in hAPOE4 knockin mice by IHC.?

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody.?

Abbr. HO, homozygous; WT, wild type.

image.png

Fig3. Detection of human APOE4 expression in Cornu ammonis 1(CA1) in hAPOE4 knockin mice by IHC.?

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody.?

Abbr. HO, homozygous; WT, wild type.

image.png

Fig4. Detection of human APOE4 expression in Cornu ammonis 3(CA3) in hAPOE4 knockin mice by IHC.?

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody.?

Abbr. HO, homozygous; WT, wild type.

image.png

Fig5. Detection of human APOE4 expression in Dentate Gyrus(DG) in hAPOE4 knockin mice by IHC.?

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody.?

Abbr. HO, homozygous; WT, wild type.

image.png

Fig6.?Blood biochemical analysis of hAPOE4 mice (female, n=3).

image.png

Fig7. Detection of Aβ Expression in Brain Tissues of hAPOE Mice.?

No specific Aβ(6E10)-positive signals were detected.?As illustrated in representative images of cortex and hippocampus, 4x

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細胞質(zhì)內(nèi)處于無活性狀態(tài)；當Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點修飾的小鼠命名，比如：敲除、敲入、點突變等等，和隨機轉(zhuǎn)基因的小鼠命名。

查看