hGUCY2C

品系全名

C57BL/6Smoc-Gucy2ctm(hGUCY2C)Smoc

目錄號

NM-HU-220417

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
Gucy2c

基因曾用名

Gcc; GC-C; AI893437

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

GUCY2C

品系描述

利用同源重組，將小鼠Gucy2c 基因進行人源化修飾。

驗證數(shù)據(jù)

image.png

Fig.1 Detection of GUCY2C expression in large intestine, small intestine and duodenum by RT-PCR.?

Wild type: only one band at 274 bp with primers F1/R1(mGucy2c);

Homozygous: only one band at 209 bp with primers F2/R2(hGUCY2C).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of GUCY2C expression in large intestine, small intestine and duodenum by qPCR.?

image.png

Fig.3 Multiple tissues of hom hGUCY2C knockin mice were stained with anti-GUCY2C antibody.?

Kidney and liver tissues served as negative controls, as the anti-human GUCY2C antibody cross-reacts with mouse and human GUCY2C. Amplifications: 200x.

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細胞質(zhì)內(nèi)處于無活性狀態(tài)；當Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點修飾的小鼠命名，比如：敲除、敲入、點突變等等，和隨機轉(zhuǎn)基因的小鼠命名。

查看