Fig1. Detection of LAG3 expression by RT-PCR.?

Wild type: only one band at 266 bp with primers 5/6(mLag3);

Heterozygous: one band at 266 bp with primers 5/6(mLAG3) and one band at 150 bp with primers 7/8(hLAG3);?

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Detection of hLAG3 expression on T cells in hLAG3 KI mice.

Note. Splenocytes from C57BL/6 and homozygous hLAG3 mice were stimulated with anti-mCD3 antibody in vitro.

圖3.? LAG3 人源化小鼠MC38 荷瘤模型體內(nèi)抗腫瘤藥效驗證。

LAG3 人源化純合子小鼠接種MC38 結(jié)腸癌細胞系，腫瘤生長至約50mm3 隨機將動物分組(n=5)。結(jié)果顯示：針對人的LAG3 抗體單獨給藥未見抗腫瘤效果，針對LAG3 抗體與PD1 抗體聯(lián)合給藥顯示出顯著的抗腫瘤效果。

圖4.?LAG3 人源化小鼠MC38 荷瘤模型體內(nèi)驗證Tencentriq 和針對LAG3 抗體聯(lián)合給藥抗腫瘤藥效。

LAG3 人源化純合子小鼠接種MC38 結(jié)腸癌細胞系，腫瘤生長至70–80 mm3 隨機分組，3 天給藥一次，連續(xù)給藥6次。