hPSGL-1(2)

品系全名

C57BL/6Smoc-Selplgem2(hSELPLG)Smoc

目錄號

NM-HU-210026

品系狀態(tài)

胚胎凍存

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基因信息

基因名
Selplg

基因曾用名

CD162; Psgl1; Selp1; Selpl; Psgl-1

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

SELPLG

品系描述

利用CRISPR基因編輯技術，將小鼠Selplg基因全部或者部分替換為人源SELPLG。從而表達人或人鼠嵌合SELPLG蛋白，取代小鼠內源Selplg蛋白的表達。
應用領域：免疫治療；藥物篩選

驗證數據

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Fig1. Detection of PSGL-1 expression in thymus and spleen by RT-PCR.?Wild type: only one band at 248 bp with primers F1/R1(mPsgl-1);Homozygous: only one band at 237 bp with primers F2/R2(hPSGL-1); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hPSGL-1 expression on B cells in blood in hPSGL-1 knockin mice.

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Fig3. Detection of hPSGL-1 expression on Monocytes and Granulocytes in blood in hPSGL-1 knockin mice.

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Fig4. Detection of hPSGL-1 expression on B cells in bone morrow in hPSGL-1 knockin mice.

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Fig5. Detection of hPSGL-1 expression on Monocytes and Granulocytes in bone morrow in hPSGL-1 knockin mice.

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Fig6. Detection of hPSGL-1 expression on T cells in bone morrow in hPSGL-1 knockin mice.

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Fig7. Detection of hPSGL-1 expression on NKs and DCs in bone morrow in hPSGL-1 KI mice.

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Fig8. Detection of hPSGL-1 expression on B cells in spleen in hPSGL-1 KI mice.

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Fig9. Detection of hPSGL-1 expression on Monocytes and Granulocytes in spleen in hPSGL-1 knockin mice.

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Fig10. Detection of hPSGL-1 expression on T cells in Thymus in hPSGL-1 knockin mice.

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