R26-rtTA/TIGRE-pTRE-hDMPK(exon 11-15)

品系全名

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-M2rtTA-WPRE-pA)Igs7em1(2xIns-DMPK 5‘region-pTRE-hDMPK(exon 11-15)-3'UTR(CTG)200-DMPK 1st intron-pA-2xIns)Smoc

目錄號

NM-XA-252292

品系狀態(tài)

精子凍存

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品系描述

通過R26-rtTA(NM-KI-232260)與TIGRE-pTRE-hDMPK(exon 11-15)(NM-KI-232261)小鼠交配獲得。

驗證數(shù)據(jù)

image.png

Fig.1 Detection of hDMPK(exon 11-15)-3'UTR(CTG)200-DMPK? expression in muscle and heart by qPCR.?

Each value in G1 and G2 represents the mRNA level relative to the value in G3(n=3).

Abbr. WT, wild type. Doxy, Doxycycline.

image.png

Fig.2 Body weight growth curve of R26-rtTA-TIGRE-DM200 (Doxy) mice. Doxy administration start from 6-8 weeks old.

Abbr. WT, Wild type; Doxy, Doxycycline.

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Fig.3 8 weeks post doxy induction, (A-C) significant skeletal muscle wasting is observed in gastrocnemius and tibialis anterior (TA) muscles from R26-rtTA-TIGRE-DM200 (Doxy) mice. (D) Tibia length is unaffected in R26-rtTA-TIGRE-DM200 (Doxy) mice compared with C57BL/6. (E) Representative image of detection of muscle loss(arrows). (n=3). *P<0.05, **P<0.01, ***P<0.001

Abbr. WT, Wild type; Doxy, Doxycycline.

image.png

Fig.4 Histopathology of C57BL6 and R26-rtTA-TIGRE-DM200 (Doxy) mice at 8 weeks post doxy induction. (A) H&E staining images of Gastrocnemius. (B) Fiber CSA (143 fibers per sample). Quantified in (C). (n=3).?

Abbr. WT, Wild type; Doxy, Doxycycline; CSA, Cross Sectional Area.

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