Fig.1 Detection of GIPR expression in brain by RT-PCR.?

Wild type: only one band at 177 bp with primers F1/R1 (mGipr);

Homozygous: only one band at 343 bp with primers F2/R2 (hGIPR).

Abbr. M, DNA marker;? HO, homozygous;? WT, wild type.

Fig. 2 Detection of human GIPR expression in hGIPR mice by WB.?

Abbr. M,marker; HO, homozygous; WT, wild type; BAT, brown adipose tissue.

Note. The anti-human GIPR Antibody cross-reacted with mouse GIPR and humanized GIPR.

Fig. 3 In vivo function of hGIP in IPGTT. Concentration of Plasma glucose were measured after intraperitoneal injection of glucose and 50 nM/kg human GIP (Cat. HY-P0276, MCE) in 6-8 weeks old wild-type C57BL/6 male mice and hGIPR male mice (n = 6); Average ± SEM; *p<0.05, **p<0.01, ***p<0.001; (Student’s t test).