Fig.1 Detection of Mrgpra3-2A-GFPCre expression in DRG, TG, brain, Spinal cord, heart, liver, spleen, lung, kidney, skin, muscle, stomach and intestine by RT-PCR.?

Mrgpra3-2A-GFPCre+ (13#): one band at 2070 bp with primers F1/R1(Mrgpra3-2A-GFPCre) in DRG and TG.?

Abbr. DRG, dorsal root ganglion; TG, trigeminal ganglia; M, DNA marker; WT, wild type.

Fig2. Cre mediated recombination in dorsal root ganglion of Mrgpra3^GFP-Cre/+;Rosa26^tdTomato/+ mouse.

Fig3. Detection of EGFP(green) and tdTomato(red) in various tissues of of Mrgpra3^GFP-Cre/+;Rosa26^tdTomato/+ mouse. The tissues of double-positive mice were cryosectioned and directly examined, revealing expression in the following organs: dorsal root ganglion.?A few cells expressed in large intestine, small intestine, thymus, adrenal gland,?testicular interstitium. The tissues that show no obvious positive signals include: brain, spinal cord, retina, heart, lungs, liver, stomach, salivary glands, brown fat, pituitary gland, kidney, spleen, pancreas, skin.

EGFP is not expressed in the thymus, while its expression pattern in other locations is similar to that of tdTomato. (53-days old, male) (For more detailed information please contact our technical advisor.)