Avil-2A-CreERT2
品系全名
C57BL/6Smoc-Avilem1(2A-CreERT2)Smoc
目錄號(hào)
NM-KI-215035
品系狀態(tài)
精子凍存
基因信息
基因名
Avil
品系描述
驗(yàn)證數(shù)據(jù)
Fig1. Avil-CreERT2/CAG-LSL-tdTomato mice were injected intraperitoneally with tamoxifen at a dose of 120 mg/kg, administered 3 times, every other day. Seven days after the last injection, the trigeminal ganglia of mice were harvested, the tdTomato signal was detected after tissue sectioning, and the NeuN signal was detected by immunofluorescence.
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Cre-ERT2在無(wú)Tamoxifen誘導(dǎo)的情況下,在細(xì)胞質(zhì)內(nèi)處于無(wú)活性狀態(tài);當(dāng)Tamoxifen誘導(dǎo)后,Tamoxifen的代謝產(chǎn)物4-OHT(雌激素類似物)與ERT結(jié)合,可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。
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