將c1型HBV病毒1.0拷貝全長DNA轉(zhuǎn)入小鼠，該轉(zhuǎn)基因小鼠血清中HBsAg呈陽性，但無HBeAg、anti-HBs、anti-HBe或antiHBc表達，無HBV DNA復制。在該轉(zhuǎn)基因小鼠模型的血清、肝臟和腎臟中可以檢測到穩(wěn)定表達的人乙肝病毒表面抗原，該小鼠模型為研究人類“乙型肝炎”的發(fā)病機制、治療方法與藥物篩選，提供了理想的動物模型和新型研究手段，具有重要的醫(yī)藥應用價值。

Fig1. Immuno-histochemical staining of HBsAg in the liver and kidney sections of #59 transgenic mice. Sections of liver (A) or kidney (B) tissues from #59 transgenic mice were stained for HBsAg.

Fig2.? Immuno-histochemical staining of HBcAg in the liver and kidney sections of #59 transgenic mice.

Sections of liver (A) or kidney (B) tissues from #59 transgenic mice were stained for HBcAg.

Tab1. Eight Selected Genes From the Kidney of #59 Mice Assayed by Microarray and Real-Time PCR

Fig3.? Dot blot immunoassay of complement C3 component in mouse serum and kidney tissue extract.

Reference

1. Ren J, Wang L, Chen Z, Ma ZM, Zhu HG, Yang DL, Li XY, Wang BI, Fei J, Wang ZG, Wen YM. Gene expression profile of transgenic mouse kidney reveals pathogenesis of hepatitis B virus associated nephropathy. J Med Virol. 2006 May;78(5):551-60. doi: 10.1002/jmv.20575. PMID: 16555286.

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