hIL2RA

品系全名

C57BL/6Smoc-Il2ratm1(hIL2RA)Smoc

目錄號

NM-HU-190064

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Il2ra

基因曾用名

CD25; Il2r; IL-2R alpha chain; Ly-43

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

IL2RA

品系描述

通過同源重組，將小鼠Il2ra基因進(jìn)行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；腫瘤研究；藥物篩選

驗(yàn)證數(shù)據(jù)

image.png

Fig1. Detection of IL2RA expression by RT-PCR.?

Wild type: only one band at 203 bp with primers F2/R2(mIl2ra);

Homozygous: only one band at 251 bp with primers F1/R1(hIL2RA);?

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.image.png

Fig2. Development and validation of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) Targeting strategy for hIL2RA knokcinmice development; (B)hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice; (C)hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice.?

image.png

Fig3. Characterization of hIL2RA knockin mice. (Completed in collaboration with CrownBio)

(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse; (B)hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse.?

image.png

Fig4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Completed in collaboration with CrownBio)

(A)Tumor growth curves upon treatment; (B) Body weight change upon treatment.?

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