hC5
品系全名
C57BL/6Smoc-Hcem1(hC5)Smoc
目錄號(hào)
NM-HU-2000013
品系狀態(tài)
活體
基因信息
基因名
品系描述
驗(yàn)證數(shù)據(jù)
Fig 1. Detection of C5 expression by RT-PCR.?
Wild type: only one band at 167 bp with primers F2/R2(mC5);
Homozygous: only one band at 337 bp with primers F1/R1(hC5).
Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig 2. Analysis of humanized C5 and humanized C5a expression in serum in the humanized C5 mouse by ELISA.?
The homozygous hC5 KI mice were treated with LPS or NS (3mg/kg, i.p.) for 6 hours.
Fig 3. Detection of hC5 expression in eyes in hC5 KI mice by IF (n=3/group).?
你也可能感興趣
Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細(xì)胞質(zhì)內(nèi)處于無活性狀態(tài)；當(dāng)Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。
查看常見的基因工程小鼠可以分為兩種命名方式，包括基因定點(diǎn)修飾的小鼠命名，比如：敲除、敲入、點(diǎn)突變等等，和隨機(jī)轉(zhuǎn)基因的小鼠命名。
查看
