4T1-hCD24

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品系基本信息

細(xì)胞系名稱

4T1-hCD24

目錄號(hào)

NM-S07-TM03

品系描述

CD24基因人源化的4T1小鼠乳腺癌細(xì)胞系

品系狀態(tài)

成瘤性驗(yàn)證完成

驗(yàn)證數(shù)據(jù)

1.?In vitro?Expression of 4T1-hCD24 cell line

The hCD24?protein expression in the 4T1-hCD24?cells were measured by Flow Cytometry. FACS data showed that significant human CD24?expression in the 4T1-hCD24?cell line.

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Figure?1. hCD24 expression in 4T1-hCD24 cell line

2.?Characterization of in vivo?growth kinetics

To test the take rates, and?the tumor growth characteristics of 4T1-hCD24?cell line, cells?were subcutaneously inoculated into the Balb/c mice. The tumor growth curve was?shown in the Fig 2.

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? ? ?Figure?2. Tumor growth curve of 4T1-hCD24 syngeneic model (n=4)


? ? ? ?image.png

Figure?3. Body weight change of 4T1-hCD24 syngeneic model (n=4)

3.?Expression of hCD24 protein in tumors

To verify the hCD24 protein expression, the tumors were dissected and seperated for single cell suspension. Robust?human CD24 protein expression can be detected in the humanized modified tumors.

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Figure?4. The expression of hCD24 in humanized modified tumors by FACS


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