Lep-KO

品系全名

B6;129S-Leptm1Smoc

目錄號

NM-KO-00034

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Lep

基因曾用名

ob; obese

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

LEP

品系描述

瘦素基因缺失小鼠是研究胰島素抵抗、肥胖、II型糖尿病等疾病的重要工具。瘦素基因的突變或缺失會導(dǎo)致嚴(yán)重的肥胖、肥胖引起的性腺功能不足，并與II型糖尿病的發(fā)展密切相關(guān)。研究還發(fā)現(xiàn)瘦素與心血管功能相關(guān)。該小鼠通常也稱為ob/ob小鼠
應(yīng)用領(lǐng)域：胰島素抵抗、肥胖、II型糖尿病等

疾病預(yù)測

2型糖尿病
Type 2 Diabetes Mellitus

近似模型的表型

參考文獻(xiàn)

Clee SM, Nadler ST, Attie AD, Genetic and genomic studies of the BTBR ob/ob mouse model of type 2 diabetes. Am J Ther. 2005 Nov-Dec;12(6):491-8

非酒精性脂肪肝
Non-Alcoholic Fatty Liver Disease

近似模型的表型

參考文獻(xiàn)

Trak-Smayra V, Paradis V, Massart J, Nasser S, Jebara V, Fromenty B, Pathology of the liver in obese and diabetic ob/ob and db/db mice fed a standard or high-calorie diet. Int J Exp Pathol. 2011 Dec;92(6):413-21

肥胖
Obesity

近似模型的表型

參考文獻(xiàn)

Barouch LA, Berkowitz DE, Harrison RW, O'Donnell CP, Hare JM, Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice. Circulation. 2003 Aug 12;108(6):754-9

腹部肥胖代謝綜合征 1
Abdominal Obesity-Metabolic Syndrome 1

近似模型的表型

參考文獻(xiàn)

Ikels K, Kuschel S, Fischer J, Kaisers W, Eberhard D, Ruther U, FTO is a relevant factor for the development of the metabolic syndrome in mice. PLoS One. 2014;9(8):e105349

腹部肥胖代謝綜合癥
Abdominal Obesity-Metabolic Syndrome

近似模型的表型

參考文獻(xiàn)

Xu A, Liu J, Liu P, Jia M, Wang H, Tao L, Mitochondrial translocation of Nur77 induced by ROS contributed to cardiomyocyte apoptosis in metabolic syndrome. Biochem Biophys Res Commun. 2014 Apr 18;446(4):1184-9

驗證數(shù)據(jù)

image.png

Fig1. Body weight and Blood glucose monitoring of ob/ob mice.

image.png

Fig2. Evaluation of Oral glucose tolerance test (OGTT) of female ob/ob mice.?

Abbr. HO, homozygous; WT, wild type. **, P ≤ 0.01.

Note. The tested ob/ob and C57BL/6 mice were 6 weeks old.

image.png


Fig3. Detection of insulin levels in serum by ELISA.?

Abbr. Hom, homozygous; HE, heterozygous; WT, wild type.

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old.

image.png

Fig4. H&E staining of the subcutaneous adipose tissue (SAT) in ob/ob mice.?

Abbr. HO, homozygous; WT, wild type; WAT, white adipose tissue; BAT, brown adipose tissue.?

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 20.

image.png

Fig5. Representative pictures of pancreas in ob/ob mice.?

These results suggested the inflammatory cell infiltration of the pancreas in ob/ob mice.?

Abbr. HO, homozygous; WT, wild type.?

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 10.

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細(xì)胞質(zhì)內(nèi)處于無活性狀態(tài)；當(dāng)Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點修飾的小鼠命名，比如：敲除、敲入、點突變等等，和隨機(jī)轉(zhuǎn)基因的小鼠命名。

查看