NCI-N87-hCLDN18.2-Tg
品系基本信息
驗證數(shù)據(jù)
Ⅰ.In vitro?Expression of NCI-N87-hCLDN18.2 cell line
The hCLDN18.2?protein expression in the wild-type NCI-N87 and NCI-N87- hCLDN18.2 cells were measured by Flow Cytometry.
Figure?1. hCLDN18.2 expression in NCI-N87 and NCI-N87-hCLDN18.2 cell lines
Ⅱ.Characterization of in vivo?growth kinetics
To test the take rates, and?the tumor growth characteristics of NCI-N87-hCLDN18.2 cell line, cells?were subcutaneously inoculated into the M-NSG mice.
Figure?2. Tumor growth curve of NCI-N87-hCLDN18.2 xenograft model (n=5)
Figure?3. Body weight change of NCI-N87-hCLDN18.2 xenograft model?model (n=5)
Ⅲ.Expression of hCLDN18.2 protein in tumors
To verify the hCLDN18.2 protein expression, the tumors were dissected and separated for single cell suspension.
Figure?4. The expression of hCLDN18.2 in humanized modified tumors by FACS
你也可能感興趣
Cre-ERT2在無Tamoxifen誘導的情況下，在細胞質(zhì)內(nèi)處于無活性狀態(tài)；當Tamoxifen誘導后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結合，可使Cre-ERT2進核發(fā)揮Cre重組酶活性。
查看
