Alpl-KO

品系全名

C57BL/6Smoc-Alpltm1Smoc

目錄號

NM-KO-18058

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
Alpl

基因曾用名

ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

ALPL

品系描述

敲除Alpl基因的exon 3-4區(qū)域，獲得Alpl基因敲除小鼠模型。

疾病預(yù)測

兒童低磷酸酯酶癥
Childhood Hypophosphatasia

近似模型的表型

參考文獻(xiàn)

Waymire KG, Mahuren JD, Jaje JM, Guilarte TR, Coburn SP, MacGregor GR, Mice lacking tissue non-specific alkaline phosphatase die from seizures due to defective metabolism of vitamin B-6. Nat Genet. 1995 Sep;11(1):45-51

嬰兒低磷酸酯酶癥
Infantile Hypophosphatasia

近似模型的表型

參考文獻(xiàn)

Narisawa S, Frohlander N, Millan JL, Inactivation of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia. Dev Dyn. 1997 Mar;208(3):432-46

驗(yàn)證數(shù)據(jù)

image.png

Fig.1 Survival of Alpl-KO mice in postnatal 15 days. (n=24 for the WT C57BL/6 group, n=50 for the HE Alpl-KO group, n=24 for the HO Alpl-KO group). (Data provided by a collaborator)?

Continuous supplementation of vitamin B6 in the diet from the pregnancy stage can significantly reduce the mortality rate caused by epilepsy in homozygous individuals.

Abbr. WT, wild type; HE, Heterozygous; HO, Homozygous.

image.png

Fig.2 Body weight of Alpl-KO mice.?

The body weight of homozygous Alpl-KO mice was markedly lower than that of both WT and heterozygous Alpl-KO mice (n=24 for the WT C57BL/6 group, n=50 for the HE Alpl-KO group, n=24 for the HO Alpl-KO group. Mean ± SEM. Two-way ANOVA , **p < 0.01, ***p < 0.001, ****p < 0.0001). (Data provided by a collaborator)??

Abbr. WT, wild type; HE, Heterozygous; HO, Homozygous.

image.png

Fig.3 Bone length of Alpl-KO mice.?

The result showed a significant reduction of the bone length in homozygous Alpl-KO mice compared with WT C57BL/6 mice at the age of 1.7-2.1 weeks (n=3 in WT C57BL/6 group, n=7 in HO Alpl-KO group. Mean ± SEM. t-test, **p < 0.01, ***p < 0.001, ****p < 0.0001). (Data provided by a collaborator)?

Abbr. WT, wild type; HO, Homozygous.

image.png

Fig.4 Body length of Alpl-KO mice.?

The body length (A) and the body length gain (B) from postnatal days 7 to 14 were both significantly decreased in homozygous Alpl-KO mice compared to WT C57BL/6 mice (n=24 for the WT C57BL/6 group, n=50 for the HE Alpl-KO group, n=20 for the HO Alpl-KO group. Mean ± SEM. Two-way ANOVA for A and One-way ANOVA for B, *p < 0.05, ****p < 0.0001, ns: no significance). (Data provided by a collaborator)?

Abbr. WT, wild type; HE, Heterozygous; HO, Homozygous.


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