Fig.1 Detection of Lmna expression in colon by RT-PCR.?

Homozygous, heterozygous and wild type: one band at 288 bp with primers F1/R1(mLmna) and one band at 123 bp with primers F/R(mGapdh).?

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig.2 The sequencing results are as expected.

Fig.3 Detection of? Lmna expression in Lmna-c.C1827T mice by WB. (Red Arrow indicates expected Progerin band)

Abbr. M, marker; WT, wild type; HO, Homozygous; HE, heterozygous; PC, positive control, Hela cells.

Fig.4 The body weight curve of WT C57BL/6 and HO Lmna(G609G) mice. (female, n=1)

Abbr. WT, wild type；HO, Homozygous

Fig.5 Representative CT scan images of WT C57BL/6 and HO Lmna (G609G) mice of 6.6 weeks. (female, n=1)

Fig.6 Representative CT scan images of WT C57BL/6 and HO Lmna (G609G) mice of 10.6 weeks. (female, n=1)

Fig.7 Representative CT scan images of WT C57BL/6 and HO Lmna (G609G) mice of 14.7 weeks. (female, n=1)

Fig.8 Relevant evaluation metrics of WT C57BL/6 and HO Lmna (G609G) mice at 6.6, 10.6, and 14.7 weeks (w) of age (female, n=1). A) Kyphosis Index (KI), B) Tibia length ), C) Skull length, D) Vertebral column length.

Fig.9 Body size of WT C57BL/6 and HO Lmna (G609G) mice. (female, n=1)

Fig.10 Representative H&E staining images of Skin/subcutaneous adipose tissue in WT and HO Lmna (G609G) mice. (female, n=1)

Fig.11 Representative H&E staining images of Aorta in WT and HO Lmna (G609G) mice. (femal, n=1)?

Fig.12 Representative H&E staining images of? Skeletal muscle in WT and HO Lmna (G609G) mice. (female, n=1)

Fig.13 Representative H&E staining images of heart in WT and HO Lmna (G609G) mice. (female, n=1)