MC38-Stk11 KO

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品系基本信息

細(xì)胞系名稱

MC38-Stk11 KO

目錄號

NMC-230070

品系描述

Stk11基因敲除的MC38小鼠結(jié)腸癌細(xì)胞系

品系狀態(tài)

成瘤性驗證完成

驗證數(shù)據(jù)

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Fig1. Stk11 expression on in vitro cultured cells of MC38-Stk11-KO.

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Fig2. In vivo validation of MC38-Stk11-KO in C57BL/6 mice. C57BL/6 mice were inoculated with MC38-Stk11-KO subcutaneously at 1×106?cells/mouse inoculum for tumor development. The day of vaccination for mice is defined as Day 0, and tumor measurement and weight measurement begin on the 3rd day after vaccination, twice a week.

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Fig3. In vivo validation of MC38-Stk11-KO in C57BL/6 mice. C57BL/6 mice were inoculated with MC38-Stk11-KO subcutaneously at 1×106 cells/mouse inoculum for tumor development. The day of vaccination for mice is defined as Day 0, and tumor measurement and weight measurement begin on the 3rd day after vaccination, twice a week.

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Fig4. Stk11 expression in tumors after in vivo growth of MC38-Stk11-KO in wild type C57BL/6 mice. When the mean tumor volume (MTV) reaches 500-800 mm3, take one tube from each group and send it to Western Blot for testing (Markers: mGapdh, mStk11); When the MTV reaches 1000-1500mm3, take 2 tubes from each group and send them to Western Blot for testing (Markers: mGapdh, mStk11). A, the MTV reaches 800 mm3. B, the MTV reaches 1500 mm3.

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