Abca4-KO

品系全名

C57BL/6Smoc-Abca4em1Smoc

目錄號(hào)

NM-KO-2104711

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Abca4

基因曾用名

RmP; Abcr; Abc10; AW050280; D430003I15Rik

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

ABCA4

品系描述

通過敲除Abca4基因exon 2，建立Abca4基因敲除小鼠模型。

疾病預(yù)測(cè)

黃斑變性疾病
Stargardt Disease

近似模型的表型

參考文獻(xiàn)

Weng J, Mata NL, Azarian SM, Tzekov RT, Birch DG, Travis GH, Insights into the function of Rim protein in photoreceptors and etiology of Stargardt's disease from the phenotype in abcr knockout mice. Cell. 1999 Jul 9;98(1):13-23

黃斑變性疾病
Stargardt Disease

近似模型的表型

MGI:6159294
注：該品系需與Rdh8-KO(NM-KO-2109660)交配才可能獲得預(yù)期表型

參考文獻(xiàn)

Wu Y, Jin Q, Yao K, Zhao J, Chen J, Wu X, Gan L, Li J, Song X, Liu X, Cai X, Retinal metabolism in humans induces the formation of an unprecedented lipofuscin fluorophore 'pdA2E'. Biochem J. 2014 Jun 15;460(3):343-52

年齡相關(guān)性黃斑變性
Age Related Macular Degeneration 2

近似模型的表型

MGI:4410294
注：該品系需與Rdh8-KO(NM-KO-2109660)交配才可能獲得預(yù)期表型

參考文獻(xiàn)

Maeda T, Maeda A, Matosky M, Okano K, Roos S, Tang J, Palczewski K, Evaluation of potential therapies for a mouse model of human age-related macular degeneration caused by delayed all-trans-retinal clearance. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4917-25

驗(yàn)證數(shù)據(jù)

image.png

Fig.1 Detection of Abca4 expression in the lung, eye, pallium, liver and kidney by qPCR.?(n=5,?3?male?mice?and?2?female?mice?in?each?group)

Abbr. HO, homozygous; WT, wild type.

image.png

Fig. 2 Detection of Cd59, Cfi, Cfh, Cfp, Cfb, C3 and C1s expression in eyes by qPCR (3 male mice in each? group, Mean ± SEM. t-test, *P < 0.05, ns: no significance).?

Abbr. HO, homozygous; WT, wild type.

image.png

Fig. 3 Detection of Cd59, Cfi, Cfh, Cfp, Cfb, C3 and C1s expression in eyes by qPCR (2 female mice in each? group, Mean ± SEM).?

Abbr. HO, homozygous; WT, wild type.

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細(xì)胞質(zhì)內(nèi)處于無活性狀態(tài)；當(dāng)Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點(diǎn)修飾的小鼠命名，比如：敲除、敲入、點(diǎn)突變等等，和隨機(jī)轉(zhuǎn)基因的小鼠命名。

查看