Fig1. Detection of PSMA expression in kidney by RT-PCR.?Wild type: only one band at 294 bp with primers F1/R1(mPsma);Heterozygous: one band at 294 bp with primers F1/R1(mPsma) and? one band at 169 bp with primers F2/R2(hPSMA); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2.?Detection of hPSMA expression in prostate in hPSMA KI mice.

Fig3. Analysis of human PSMA expression in the MC38-hPSMA cell line and the transplanted tumor of the PSMA humanized mice by FACS.?Most of the MC38-hPSMA cells express human PSMA (A,B);? the transplanted tumor formed by MC38-hPSMA cell line expresses human PSMA on the CD45- cells (C).(Completed in collaboration with CrownBio)

Fig4. Subcutaneous tumor formation experiment of the MC38-hPSMA cells in the PSMA humanized mice .The tumor volume was measured every 5 days after injection of MC38-hPSMA cells (n=10).The previous studies have shown that wild type mice would spontaneously reject the syngeneic tumors? expressing an introduced human tumor antigen-hPSMA. While the MC38-hPSMA cells can form tumors subcutaneously in the humanized PSMA mice, indicating that the PSMA humanized mouse model can be used to transplant the MC38-hPSMA cell line.(Completed in collaboration with CrownBio)

Fig5. Detection of human PSMA expression in Prostate by IHC.

Note. The human PSMA antibody cross-reacted with mouse PSMA and human PSMA.??

Abbr. HO, homozygous; WT, wild type.