hGLP1R

品系全名

C57BL/6Smoc-Glp1rem2(hGLP1R)Smoc

目錄號

NM-HU-200220

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Glp1r

基因曾用名

GLP-1R; GLP1Rc

染色體位置

17

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

GLP1R

品系描述

利用CRISPR基因編輯技術(shù)，將小鼠Glp1r基因全部或者部分替換為人源hGLP1R，從而表達(dá)人或人鼠嵌合hGLP1R蛋白，取代小鼠內(nèi)源Glp1r蛋白的表達(dá)。
應(yīng)用領(lǐng)域：糖尿病研究；藥物篩選

驗證數(shù)據(jù)

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Fig 1. Detection of GLP1R and?Gapdh expression in lung, heart, brain, stomach, salivary gland, duodenum and samll intestine by RT-PCR.

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Fig 2. Analysis of hGLP1R expression by IHC.?Pancreata from wild-type and hGLP1R mice (HO) were stained using an antibody specific for human GLP1R, and only the pancreas from hGLP1R mice (HO) showed hGLP1R positive signal in pancreatic islet.

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Fig 3. Assessment of biochemical parameters in HO hGLP1R mice after high-fat diet feeding. (A) Oral glucose tolerance test (OGTT) in hGLP1R mice after 3 months high-fat diet feeding (n = 5/group); (B) Blood biochemical analysis in hGLP1R mice after 6 months high-fat diet feeding?(n = 5/group).

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Fig 4. Validation of the hypoglycemic efficacy of the test compound in humanized GLP-1R mice. The oral glucose tolerance test (OGTT) was performed (n=5), and the test compound showed a better hypoglycemic efficacy (p<0.001). The above data indicated that the GLP-1R humanized mouse is an effective model for diabetes drug screening.

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Fig 5. Efficacy study of PF06882961 in hGLP1R mice. PF06882961 has shown significant effect in hGLP1R mice in OGTT study, but no effect in C57BL/6 mice.

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Fig 6. Efficacy study of PF06882961 in HFD/STZ induced type II diabetes model in hGLPIR mice. PF06882961 could significant decreases the body weight(A); PF06882961 could significantly decrease fast blood glucose level and AUC in OGTT study (B).

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Fig 7. Detection of human GLP-1R expression in HO hGLP-1R knockin mice by IHC.?Pancreas, salivary gland, colon, heart, lung, brian, stomach and duodenum were collected from homozygous hGLP-1R mice (HO/HO), and analyzed by IHC with anti-human GLP-1R antibody. Human GLP-1R is specifically expressed in islets of the pancreas. Abbr. HO/HO, homozygous.

Table1. Blood routine test results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).

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Table2. Biochemistry examinations results of Hom hGLP-1R mice (Data are presented as mean and ± SEM).

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發(fā)表文獻(xiàn)

GLP-1R mediates idebenone-reduced blood glucose in mice
來源雜志：Biomedicine &amp; Pharmacotherapy

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