hFGFR3-G380R

品系全名

C57BL/6Smoc-Fgfr3tm1(hFGFR3-G380R)Smoc

目錄號(hào)

NM-HU-232149

品系狀態(tài)

精子凍存

導(dǎo)出PDF

基因信息

基因名
Fgfr3

基因曾用名

FR3; Mfr3; sam3; CD333; Flg-2; HBGFR; Fgfr-3

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

FGFR3

品系描述

利用同源重組，將小鼠Fgfr3基因進(jìn)行人源化G380R突變修飾。

驗(yàn)證數(shù)據(jù)

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Fig.1 Detection of FGFR3 expression in knee joint and brain in 2.4-week-old male and female hFGFR3-G380R knockin mice by RT-PCR.?

HepG2 cells were used as the WT control of human FGFR3 RNA.?

Wild type: one band at 217 bp with primers F1/R1(mFgfr3) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 217 bp with primers F1/R1(mFgfr3), one band at 375 bp with primers F2/R2(hFGFR3) and one band at 123 bp with primers F/R(mGapdh).?

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

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Fig.2 Sequencing analysis of human FGFR3 mRNA in HepG2 cells and hFGFR3-G380R knockin mice.?

Sequencing data of RT-PCR products confirmed the presence of a G to A mutation in human FGFR3 mRNA expressed in hFGFR3-G380R knockin mice.?

Abbr. HE, heterozygous.

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Fig.3 Body weight of WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice. (n=1)

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Fig.4 Micro-CT analysis of skeleton in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.5 Micro-CT analysis of skeleton in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.6 Micro-CT analysis of skeleton in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.7 Micro-CT analysis of skeleton in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

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Fig.8 Micro-CT analysis of femur in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.9 Micro-CT analysis of femur in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.10 Micro-CT analysis of femur in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.11 Micro-CT analysis of femur in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

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Fig.12 Micro-CT analysis of skull in 35-day-old? WT C57BL/6 and heterozygous hFGFR3-G380R mice.

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Fig.13 Micro-CT analysis of skull in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

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Fig.14 Micro-CT analysis of skull in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

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Fig.15 Micro-CT analysis of skull in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

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