hIL1RL1/hIL4/hIL4R

品系全名

C57BL/6Smoc-Il1rl1tm4(hIL1RL1)Il4tm3(hIL4)Il4ratm1(hIL4R)Smoc

目錄號(hào)

NM-HU-232112

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

品系描述

通過(guò)hIL4/hIL4R(NM-HU-2000106)與hIL1RL1(NM-HU-2000067)小鼠交配獲得。

驗(yàn)證數(shù)據(jù)

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Fig1. Expression of hIL1RL1 on CD4+ T cells in spleen in hIL1RL1/hIL4/hIL4R tKI mice.

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Fig2. Expression of hIL1RL1 on mast cells in bone marrow in hIL1RL1/hIL4/hIL4R tKI mice.

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Fig3. Detection of soluble form hIL4RA (A) and mIL4RA (B) expression in hIL4RA mice by ELISA.?

Abbr. HO: homozygote; WT: Wild type; ND: Not detected.

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Fig4. Analysis of hIL4R expression in the spleen in hIL4/hIL4R dKI mice by FACS.?

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Fig5. Evaluation of an Anti-huIL4Ra mAb in the OXA-Induced Atopic Dermatitis Model in hIL4/hIL4Ra dKI Mice.?

Both dupilumab and Dexamethasone ameliorate overall atopic dermatitis activity in OXA - challenged ears, but only Dexamethasone worked in back skin. (In cooperation with CrownBio)

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Fig6. Dupilumab significantly mitigates inflammatory cell infiltration in lesioned ear. (In cooperation with CrownBio)?

Note: Inflammatory cell infiltration is indicated by black arrows, 10X images

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Fig7. Low dose dupilumab significantly mitigates inflammatory cell infiltration in lesioned skin.??(In cooperation with CrownBio)?

Note: Inflammatory cell infiltration is indicated by black arrows, 10X images

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Fig8. Dexamethasone and dupilumab treatments are well tolerated. Both significantly reduced IgE levels in serum but not spleen weight.?(In cooperation with CrownBio)?

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.

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Fig9. Repeated OXA-induced Th2 cytokine production (hIL-4 elevation) and dupilumab treatment significantly reduces IL-6 and KC/GRO levels in lesioned ear.?(In cooperation with CrownBio)?

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.

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Fig10. Repeated OXA-induced Th2 cytokine production (hIL-4 elevation) and dupilumab treatment significantly reduces IL-5 and IL-2 levels in lesioned skin.?(In cooperation with CrownBio)?

Ordinary one-way ANOVA were applied for analysis compared to Isotype group, *p<0.05, **p<0.01, *** p<0.001.

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