hCD3EDG(BALB/c)

品系全名

BALB/c-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)/CbSmoc

目錄號

NM-HU-220122

品系狀態(tài)

活體

導(dǎo)出PDF

品系描述

通過同源重組，對小鼠Cd3e/Cd3d/Cd3g基因進行人源化修飾。

驗證數(shù)據(jù)

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Fig1. Detection of surface expression of human CD3E/CD3D on T cells in spleen in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)

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Fig2. Detection of surface expression of human CD3E/CD3G on T cells in blood in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)

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Fig3. Analysis of TCR signalling in hCD3EDG (BALB/c) mice upon CD3/CD28 activation in vitro. (In collaboration with CrownBio.)

Note: CFSE-dilution as a measure of mouse T cell proliferation.

CD3+ T cells were isolated in the splenocytes from WT BALB/c and hCD3EDG mice. Isolated T cells were labeled with μCFSE dye and stimulated with anti-mCD3 (5μg/mL) or anti-hCD3 (5μg/mL) plus soluble anti-mCD28 (50μg/mL) for in vitro culture for 72 hr. T cell proliferation was analyzed by flow cytometry for CFSE dilution.?

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