hIL12B

品系全名

C57BL/6Smoc-Il12betm3(hIL12B)/Smoc

目錄號

NM-HU-2000057

品系狀態(tài)

胚胎凍存

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基因信息

基因名
Il12b

基因曾用名

p40， Il-12b， Il12p40， Il-12p40

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

IL12B

品系描述

通過同源重組，將小鼠Il12b基因進行人源化修飾。
應用領域：細胞因子人源化模型；藥物篩選

驗證數(shù)據(jù)

image.png

Fig1. Detection of IL12B expression in thymus by RT-PCR.?

Wild type: only one band at 260 bp with primers F1/R1(mIl12b);

Heterozygous: one band at 260 bp with primers F1/R1(mIl12b) and one band at 260 bp with primers F2/R2(hIL12B);?

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. Detection of hIL12B expression in plasma by ELISA.?

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.?

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

image.png

Fig 3. Detection of mIL12B expression in plasma by ELISA.?

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.?

Note. hIL12B and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 2h.

image.png

Fig4. Detection of human IL12P70 and mouse IL12P70 expression in serum by ELISA (n=3).?

Abbr. HO, homozygous; WT, wild type.

Note. hIL12A/hIL12B and C57BL/6 mice were i.p. injected with LPS for 2h.

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