Fig1. Detection of NT5E expression in liver and lung by RT-PCR.?Wild type: only one band at 221 bp with primers F1/R1(mNt5e);Homozygous: only one band at 285 bp with primers F2/R2(hNT5E); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig2. Expression of CD73 in the? peripheral T cells collected from humanized CD73 mice were detected by FACS.?The results showed that the expression of human CD73 can be detected in the? peripheral T cells of humanized CD73 mice as same as in Hu-PBMC.

Fig.3?In vivo?validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized CD73 mice.?Homozygous humanized CD73 mice were inoculated with MC38 colon cancer cells (expressing human CD73 rather than murine CD73). Mice were grouped when the tumor size was approximately 100 mm3(n=6). Three human CD73 antibodies differently inhibited tumor growth, confirming that the CD73-HU mouse model is a powerful tool for in vivo CD73 antibody pharmacological efficacy study. A.Tumor average volume ± SEM, B.Mice average weight ± SEM.