hCD3EDG/hDLL3

品系全名

C57BL/6Smoc-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Dll3em1(hDLL3) /Smoc

目錄號

NM-XA-240036

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Dll3

基因曾用名

pu; pudgy

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

DLL3

品系描述

通過hDLL3(NM-HU-200231)與hCD3EDG(NM-HU-220120)小鼠交配獲得。

驗證數(shù)據(jù)

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Fig.1 Detection of humanized DLL3 expression in male hCD3EDG/hDLL3 mice by RT-PCR.?

Homozygous:? one band at 282 bp with primers F2/R2(hDLL3).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of humanized DLL3 expression in female hCD3EDG/hDLL3 mice by RT-PCR.?

Homozygous: one band at 282 bp with primers F2/R2(hDLL3).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of mouse Dll3 expression in male hCD3EDG/hDLL3 mice by RT-PCR.?

Wild type: one band at 172 bp with primers F1/R1(mDll3).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.4 Detection of mouse Dll3 expression in female hCD3EDG/hDLL3 mice by RT-PCR.

Wild type: one band at 172 bp with primers F1/R1(mDll3).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.5 Detection of mouse Gapdh expression in male hCD3EDG/hDLL3 mice by RT-PCR.?

Wild type and homozygous: one band at 123 bp with primers F/R(mGapdh).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.6 Detection of mouse Gapdh expression in female hCD3EDG/hDLL3 mice by RT-PCR.?

Wild type and homozygous: one band at 123 bp with primers F/R(mGapdh).?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.7 Detection of human DLL3 expression in 5 embryos (E10.5~E14.5), whose parents are both HO hCD3EDG/hDLL3 mice, by WB.?

Abbr. HO, homozygous.

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Fig.8 Detection of human DLL3 expression in 7-14-week-old hCD3EDG/hDLL3 knockin mice by WB.?

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Fig.9 Detection of (A) body weight, (B) the weights of different tissues and (C) organ index in HO hCD3EDG/hDLL3 mice.

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Fig.10 Detection of mIgG(A)? and mIgM(B) expression in serum by ELISA.?

Abbr. HO, homozygous; WT, wild type.

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Fig.11 Detection of immune phenotype in (A) peripheral blood and (B) spleen by FACS.??

Abbr. HO, homozygous; WT, wild type.


Table 1. Blood routine examination in hCD3EDG/hDLL3 dKI mice (n=3 /group).

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