hCA9
品系全名
C57BL/6Smoc-Car9tm1(hCA9)Smoc
目錄號
NM-HU-234371
品系狀態(tài) 活體
基因信息
基因名
Car9
品系描述
利用同源重組，將小鼠Car9基因進行人源化修飾。
*使用本品系發(fā)表的文獻需注明: hCA9 mice (Cat. NO. NM-HU-234371) were purchased from Shanghai Model Organisms Center, Inc..
驗證數(shù)據(jù)
Fig.1 Detection of CA9 expression in stomach by RT-PCR.?
Wild type: only one band at 144 bp with primers F1/R1(mCar9);
Homozygous: only one band at 186 bp with primers F2/R2(hCA9).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human CA9 expression in hCA9 knockin mice by WB.?
Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, HCT116 cells.
Note. The anti-human CA9 antibody cross-reacted with mouse CA9 and humanized CA9. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.
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