Th-IRES-CreERT2
品系全名
C57BL/6Smoc-Them1(IRES-creER)Smoc
目錄號(hào)
NM-KI-200082
品系狀態(tài)
活體
基因信息
基因名
Th
品系描述
驗(yàn)證數(shù)據(jù)
Fig1. Cre mediated recombination in brain of ThCreERT2/+;Rosa26tdTomato/+ mouse. (VTA: ventral tegmental area)
Fig2. Detection of tdTomato(red) in various tissues of ThCreERT2/+;Rosa26tdTomato/+ mouse. Expression tissues include: brain, islet. A few cells expressed in spinal cord, pituitary gland, small intestine, colon, skin, thymus and adrenal gland. Tissues that unexpressed include eye, heart, lung, liver, stomach, salivary glands, brown fat, kidney, spleen, muscle. Tamoxifen induction began at 49 days of age, with testing at 58 days. (For more detailed information please contact our technical advisor.)?
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Cre-ERT2在無(wú)Tamoxifen誘導(dǎo)的情況下,在細(xì)胞質(zhì)內(nèi)處于無(wú)活性狀態(tài);當(dāng)Tamoxifen誘導(dǎo)后,Tamoxifen的代謝產(chǎn)物4-OHT(雌激素類似物)與ERT結(jié)合,可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。
查看常見的基因工程小鼠可以分為兩種命名方式,包括基因定點(diǎn)修飾的小鼠命名,比如:敲除、敲入、點(diǎn)突變等等,和隨機(jī)轉(zhuǎn)基因的小鼠命名。
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