Rpe65-IRES-CreERT2
品系全名
C57BL/6Smoc-Rpe65em1(IRES-CreERT2-pA)Smoc
目錄號
NM-KI-242372
品系狀態(tài) 精子凍存
基因信息
基因名
Rpe65
品系描述
將IRES-CreERT2-pA插入到小鼠Rpe65基因終止密碼子處。
*使用本品系發(fā)表的文獻需注明: Rpe65-IRES-CreERT2 mice (Cat. NO. NM-KI-242372) were purchased from Shanghai Model Organisms Center, Inc..
驗證數(shù)據(jù)
Fig1. CreERT2 mediated recombination in retina of Rpe65 CreERT2/+;Rosa26 tdTomato/+ mice.
Fig2. Detection of tdTomato(red) in various tissues of Rpe65 CreERT2/+;Rosa26 tdTomato/+ mice. After tamoxifen was induced, the organization of tdTomato expression: retina. Tissues that do not express tdTomato: brain, lung, kidney, reproductive system. (32-days old, female) (For more detailed information please contact our technical advisor.)?
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Tamoxifen誘導Cre-ERT2小鼠 使用指南
Cre-ERT2在無Tamoxifen誘導的情況下，在細胞質(zhì)內(nèi)處于無活性狀態(tài)；當Tamoxifen誘導后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進核發(fā)揮Cre重組酶活性。
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