Tnf-KO

品系全名

C57BL/6Smoc-Tnfem1Smoc

目錄號

NM-KO-00100

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名
Tnf

基因曾用名

DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

TNF

品系描述

Tnf基因位于小鼠17號染色體。設(shè)計(jì)sgRNA針對Tnf基因exon 2-3，建立該Manf基因敲除小鼠模型
應(yīng)用領(lǐng)域：免疫相關(guān)研究；腫瘤相關(guān)研究

疾病預(yù)測

脊柱關(guān)節(jié)病
Spondyloarthropathy

近似模型的表型

參考文獻(xiàn)

Sakkou M, Chouvardas P, Ntari L, Prados A, Moreth K, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Denis MC, Karagianni N, Kollias G, Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis. JCI Insight. 2018 Apr 5;3(7)

類風(fēng)濕關(guān)節(jié)炎
Rheumatoid Arthritis

近似模型的表型

參考文獻(xiàn)

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

類風(fēng)濕關(guān)節(jié)炎
Rheumatoid Arthritis

近似模型的表型

參考文獻(xiàn)

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

驗(yàn)證數(shù)據(jù)

image.png

Fig.1 Detection of TNF expression in spleen and thymus by RT-PCR.?

Wild type: only one band at 209 bp with primers F1/R1(mTnf).?

Abbr. M, DNA marker;? HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of mTnf expression in spleen and thymus by Q-PCR.?

image.png

Fig.3 Detection of mTNF-a expression in serum by ELISA (male, 8wks, n=2).?

Abbr. HO, Homozygous; WT, wild type.

Note.TNF-KO and C57BL/6 mice were i.p. injected with LPS .

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