hIL33

品系全名

C57BL/6Smoc-Il33tm2(hIL33)/Smoc

目錄號

NM-HU-2000069

品系狀態(tài)

胚胎凍存

導出PDF

基因信息

基因名
Il33

品系描述

通過同源重組,將小鼠Il33基因進行人源化修飾。
應用領(lǐng)域:細胞因子人源化模型;藥物篩選

驗證數(shù)據(jù)

image.png

Fig1. Detection of IL33 expression in lung by RT-PCR.?Wild type: only one band at 287 bp with primers F1/R1(mIl33);Homozygous: only one band at 311 bp with primers F2/R2(hIL33); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig 2. Detection of hIL33 expression in lung homogenate by ELISA.?Abbr. HO, homozygous; HE, heterozygous; WT, wild type.Note. hIL33 and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 4h.

image.png

Fig 3. Detection of mIL33 expression in lung homogenate by ELISA.?Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS(10mg/kg) for 4h.


你也可能感興趣

Tamoxifen誘導Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導的情況下,在細胞質(zhì)內(nèi)處于無活性狀態(tài);當Tamoxifen誘導后,Tamoxifen的代謝產(chǎn)物4-OHT(雌激素類似物)與ERT結(jié)合,可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式,包括基因定點修飾的小鼠命名,比如:敲除、敲入、點突變等等,和隨機轉(zhuǎn)基因的小鼠命名。

查看