hIL18BP

品系全名

C57BL/6Smoc-Il18bpem1(hIL18BP)Smoc

目錄號(hào)

NM-HU-210039

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
Il18bp

基因曾用名

MC54L; Igifbp; IL-18BP

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

IL18BP

品系描述

通過同源重組，將小鼠Il18bp基因進(jìn)行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；藥物篩選

驗(yàn)證數(shù)據(jù)

image.png

Fig1. Detection of IL18BP expression in thymus and liver by RT-PCR.?Wild type: only one band at 354 bp with primers F1/R1(mIl18bp);Heterozygous: one band at 354 bp with primers F1/R1(mIl18bp) and one band at 220 bp with primers F2/R2(hIL18BP); Abbr.. M, DNA marker;?HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig2. Detection of IL18BP expression in thymus and liver by RT-PCR.? Wild type: only one band at 354 bp with primers F1/R1(mIl18bp); Homozygous: only one band at 220 bp with primers F2/R2(hIL18BP);? Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig3. Detection of hIL18BP expression in serum by ELISA.?Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig4. Detection of mIL18BP expression in serum by ELISA.?Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig5.?Detection of hIL18BP expression in serum by ELISA (n=3).?Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

image.png

Fig6.?Detection of mIL18BP expression in serum by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下，在細(xì)胞質(zhì)內(nèi)處于無活性狀態(tài)；當(dāng)Tamoxifen誘導(dǎo)后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進(jìn)核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點(diǎn)修飾的小鼠命名，比如：敲除、敲入、點(diǎn)突變等等，和隨機(jī)轉(zhuǎn)基因的小鼠命名。

查看