hCSF1/hCSF1R(BALB/c)

品系全名

BALB/cAnSmoc-Csf1tm2(CSF1)Csf1rem3(hCSF1R)/Smoc

目錄號(hào)

NM-HU-220016

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名

品系描述

通過同源重組,將小鼠Csf1和Csf1r基因進(jìn)行人源化修飾。

驗(yàn)證數(shù)據(jù)

image.png

Fig.1?Detection?of?CSF1?expression?in?spleen?and?lung?by?RT-PCR.?

Wild type: only one band at 299 bp with primers F1/R1(mCsf1);

Homozygous: only one band at 261 bp with primers F2/R2(hCSF1);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of CSF1R expression in spleen and lung by RT-PCR.?

Wild type: only one band at 233 bp with primers F1/R1(mCsf1r);

Homozygous: only one band at 345 bp with primers F2/R2(hCSF1R);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hCSF1 expression in serum by ELISA (n=3).?

Abbr. HO, homozygous; WT, wild type.

Note. hCSF1/hCSF1R and BALB/c mice were i.p. injected with LPS for 6h.

image.png

Fig.4 Expression characterization of hCSF1R on Monocytes/ macrophages in Bone marrow in hCSF1/hCSF1R dKI mice.



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