hTNFR2

品系全名

C57BL/6Smoc-Tnfrsf1bem2(hTNFRSF1B)Smoc

目錄號(hào)

NM-HU-190010

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
TNFRSF1B

基因曾用名

p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

TNFRSF1B

品系描述

通過同源重組，將小鼠Tnfrsf1b基因進(jìn)行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；腫瘤研究；藥物篩選

驗(yàn)證數(shù)據(jù)

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Fig.1 Detection of TNFRSF1B expression by RT-PCR.?

Wild type: only one band at 247 bp with primers F2/R2(mTnfrsf1b);

Homozygous: only one band at 255 bp with primers F1/R1(hTNFRSF1B);?

Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig.2 Detection of human/mouse TNFRSF1B(TNFR2) expression on Treg, granulocytes and monocytes in spleen in HE hTNFR2 mice.（In collaboration with CrownBio）

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Fig.3 Detection of human/mouse TNFRSF1B(TNFR2) expression on Treg, granulocytes and monocytes in Peripheral Blood in HE hTNFR2 mice.（In collaboration with CrownBio）

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Fig.4 Detection of human/mouse TNFRSF1B(TNFR2) expression on Treg, granulocytes and monocytes in Lymph node in HE hTNFR2 mice.（In collaboration with CrownBio）

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Fig.5 Detection of human/mouse TNFRSF1B(TNFR2) expression on T cells in spleen in HO hTNFR2 mice.（In collaboration with CrownBio）

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Fig.6 Detection of human/mouse TNFRSF1B(TNFR2) expression on Treg, granulocytes and monocytes in spleen in HO hTNFR2 mice.（In collaboration with CrownBio）

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Fig.7?In vivo efficacy evaluation of anti-hTNFR2 in TNFR2 HuGEMM mice with MC38-OVA tumors.（In?collaboration with CrownBio）

Homozygous humanized TNFRSF1B mice were inoculated with MC38 colon cancer cells. The drug targeting human TNFRSF1B, showed a very significant anti-tumor effect, demonstrating that the humanized TNFRSF1B mice are a good in vivo model for validating the efficacy of antibodies targeting human TNFRSF1B.

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Fig.8?FACS for blood upon TNFR2 antagonist treatment.（In?collaboration with CrownBio）

Addition of antagonistic hTNFRSF1B antibody can impair hTNF-induced Treg cells proliferation, while the agonistic hTNFRSF1B antibody alone can induce a modest proliferative response.

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Fig.9?TIL analysis upon TNFR2 antagonist treatment.（In?collaboration with CrownBio）

1 tumor in hTNFR2 Ab1 treatment group decreased? (1/7, TV=0)

2 tumors in hTNFR2 Ab2 treatment group decreased? (2/7, TV=0; 3/7 died during dosing period)

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Fig.10?TNFRSF1B blockade increases the Teff/Treg ratios in tumor microenvironment.?（In?collaboration with CrownBio）

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