hCFB

品系全名

C57BL/6Smoc-Cfbtm1(hCFB)/Smoc

目錄號

NM-HU-2000078

品系狀態(tài)

活體

導出PDF

基因信息

基因名
Cfb

基因曾用名

Bf; C2; Fb; H2-Bf

染色體位置

17

NCBI ID

MGI ID

Ensembl ID

Pubmed

品系描述

通過同源重組，將小鼠Cfb基因進行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；藥物篩選

驗證數(shù)據(jù)

image.png

Fig1. Detection of CFB expression in liver by RT-PCR.?

Wild type: only one band at 441 bp with primers F1/R1(mCfb);

Homozygous: only one band at 442 bp with primers F2/R2(hCFB);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig2. Detection of human and mouse CFB expression in serum by ELISA (n=2).?

Abbr. HO, homozygous; WT, wild type.

Note: The mouse CFB ELISA kit cross-reacted with mCFB and humanized CFB.

image.png

Fig3. Detection of hCFB expression in serum by ELISA(n=3/group).?

Abbr. HO, homozygous; WT, wild type

image.png

Fig4. Body Weight(A) and Survival Curve(B) of WT C57BL/6 and HO hCFB mice.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig5. Blood Biochemistry of WT C57BL/6 and HO hCFB mice.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig6. Detection of liver CFB mRNA remaining at Day 7 (A) and serum protein level (B) after single dose of test drug (n=3/group).?(Data from a collaborator)

image.png

Fig7.Partial representative pictures of liver and kidney pathology in hCFB knockin mice.?No obvious lesions were observed in hCFB knockin mice.?

你也可能感興趣

Tamoxifen誘導Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導的情況下，在細胞質(zhì)內(nèi)處于無活性狀態(tài)；當Tamoxifen誘導后，Tamoxifen的代謝產(chǎn)物4-OHT（雌激素類似物）與ERT結(jié)合，可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式，包括基因定點修飾的小鼠命名，比如：敲除、敲入、點突變等等，和隨機轉(zhuǎn)基因的小鼠命名。

查看