hMASP2

品系全名

C57BL/6Smoc-Masp2em1(hMASP2)/Smoc

目錄號(hào)

NM-HU-2000076

品系狀態(tài)

活體

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基因信息

基因名
Masp2

基因曾用名

MAp19; MASP-2

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

MASP2

品系描述

通過(guò)同源重組，將小鼠Masp2基因進(jìn)行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；藥物篩選

驗(yàn)證數(shù)據(jù)

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Fig1. Detection of MASP2 expression in liver by RT-PCR.?

Wild type: only one band at 479 bp with primers F1/R1(mMasp2);

Homozygous: only one band at 338 bp with primers F2/R2(hMASP2);?

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

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Fig2. Detection of hMASP2 expression in liver in HO hMASP2 mice by western blot.

Abbr. HO, homozygous; WT, wild type.

Note: The anti-human MASP2 antibody cross-reacted with mouse MASP2 and humanized MASP2.

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Fig3. Detection of hMASP2 expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig4. Detection of hMASP2 expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig5. Detection of IgA expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig6. Detection of IgA expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

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Fig7. No obvious pathological features were found in liver in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig8. No obvious pathological features were found in kidney in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig9. Detection of mC3 expression in serum by ELISA.?

Abbr. HO, homozygous; WT, wild type.

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Fig10. Lectin and alternative pathway activity of HO hMASP2 mice.?

LP activity in serum of age-matched female (A) and male (B) wild-type C57BL/6 and HO hMASP2 mice at different dilutions (n=3/group); (C) AP activity in serum of age-matched male wild-type?C57BL/6 and HO hMASP2?mice at different dilutions (n=3/group).

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Fig11. LNP023 blocks alternative complement pathway activity in the serum of age-matched male wild-type C57BL/6 and HO hMASP2 mice (n=3/group).

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