hC5AR1/C5AR2

品系全名

C57BL/6Smoc-C5ar1tm1(hC5AR1)C5ar2tm1(hC5AR2)/Smoc

目錄號

NM-HU-2000018

品系狀態(tài)

活體

導(dǎo)出PDF

基因信息

基因名

基因曾用名

C5aR C5r1 Cd88 D7Msu1，C5L2， Gpr77， E030029A11Rik

NCBI ID

MGI ID

Pubmed

人類同源基因

C5AR1，C5AR2

品系描述

通過同源重組，將小鼠C5ar1和C5ar2基因進行人源化修飾。
應(yīng)用領(lǐng)域：免疫治療；藥物篩選

驗證數(shù)據(jù)

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Fig1. Detection of C5AR1 and?C5AR2?expression in spleen by RT-PCR.?

Wild type: only one band at 257 bp with primers F1/R1 (mC5ar1), only one band at 506 bp with primers F3/R3 (mC5ar2);

Homozygous: only one band at 313 bp with primers F2/R2 (hC5AR1),??only one band at 318 bp with primers F4/R4 (hC5AR2).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.image.png

Fig2. Detection of C5AR1 expression on myeloid cells in blood in?hC5AR1 knockin mice.

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Fig3. Detection of C5AR1 expression on myeloid cells in bone morrow in hC5AR1 knockin mice.?

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Fig4.?Detection of C5AR2 expression on myeloid cells in spleen in hC5AR1/hC5AR2 dKI mice.?(Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

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Fig5.?Detection of C5AR2 expression on myeloid cells in bone marrow in hC5AR1/hC5AR2 dKI mice.?(Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

image.png

Fig6. Detection of hC5AR2 expression on myeloid cells?in peritoneal lavage fluids in?hC5AR1/hC5AR2 dKI mice.?(Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

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