H22-Luc-hGPC3
品系基本信息
驗(yàn)證數(shù)據(jù)
1. In vitro?Expression of H22-Luc-hGPC3 cell line
hGPC3 expression was measured in H22-Luc-hGPC3 cell?by?FACS?. FACS results showed that significant hGPC3?expression on the H22-Luc-hGPC3?cell?line.
Figure?1 hGPC3 expression in H22-Luc-hGPC3 cell line
2. Characterization of in vivo?growth kinetics
To verify tumor formation of?H22-Luc-hGPC3?cell line,?cells?were subcutaneously inoculated into?female?balb/c?mice.
Figure?2. Tumor growth curve of H22-Luc-hGPC3 allogeneic model (n=5)
Figure 3. Body weight curve of H22-Luc-hGPC3 allogeneic model (n=5)
3. Expression of hGPC3 in tumors
To verify the hGPC3 protein expression, the tumors were dissected and separated for single cell suspension when he tumor volume is 500-1000mm3. Robust hGPC3 protein expression can be detected in the humanized modified tumors.
Figure?4. The expression of hGPC3 in humanized modified tumors by FACS
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