E.G7-OVA-hCD24
品系基本信息
驗證數(shù)據(jù)
Ⅰ.In vitro?Expression of E.G7-OVA-hCD24-Luc cell line
The human CD24 protein expression in the E.G7-OVA and E.G7-OVA-hCD24-Luc cells were measured by Flow Cytometry.
Figure?1. hCD24 expression in wild-type E.G7-OVA and E.G7-OVA-hCD24-Luc cell lines
Luciferase activity?expression on the E.G7-OVA-hCD24-Luc?cell?was measured by microplate reader.
Figure 2. Luciferase activity assay of E.G7-OVA-hCD24-Luc cell line
Ⅱ.Characterization of in vivo?growth kinetics
To verify whether there is the?difference in tumor formation between the E.G7-OVA -hCD24-Luc?cell line and the E.G7-OVA?cell line, both cells?were subcutaneously inoculated into C57BL/6 mice.
Figure?3. Tumor growth curve of E.G7-OVA and E.G7-OVA-hCD24-Luc syngeneic model
Figure?4. Body weight change of E.G7-OVA and E.G7-OVA-hCD24-Luc?syngeneic model
Ⅲ.?Expression of OVA protein in tumors
To verify the human CD24 protein expression, the tumors were dissected and separated for single cell suspension.
Figure?5. The expression of hCD24 in humanized modified tumors by FACS
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