Cd8a-2A-tdTomato-2A-Luc

品系全名

C57BL/6Smoc-Cd8aem(T2A-tdTomato-P2A-Luciferase)1Smoc

目錄號

NM-KI-225025

品系狀態(tài)

精子凍存

導(dǎo)出PDF

基因信息

基因名
Cd8a

品系描述

將T2A-tdTomato-P2A-Luciferase插入到小鼠Cd8a基因終止密碼子處。
應(yīng)用領(lǐng)域:熒光示蹤小鼠

驗證數(shù)據(jù)

Fig.1.png

Fig.1?IVIS automatic exposure.According to the imaging process, the mice were injected with D-Luciferin, and the abdomen, back, and flanks were imaged in vivo.?

image.png

Fig.2?IVIS automatic exposure: ex vivo.?According to the imaging process, D-Luciferin was injected, and the mouse brain, heart, lung, kidney, liver, ?spleen, thymus, inguinal lymph node, axial lymph node (Axial LN), muscle, and the entire digestive system ?(stomach to colon) were taken for imaging.

Fig.3.pngFig.3?Detection of tdTomato in mice thymus by FACS.

Fig.4.png

Fig.4?Detection of tdTomato in mice axillary lymph by FACS.

Fig.5.png

Fig.5?Detection of tdTomato in mice inguinal lymph by FACS.

Fig.6.pngFig.6 Detection of tdTomato in mice spleen by FACS.

Fig.7.pngFig.7?Detection of tdTomato in mice PB by FACS.?

*The validation data above?were completed in cooperation with Crownbio.


你也可能感興趣

Tamoxifen誘導(dǎo)Cre-ERT2小鼠 使用指南

Cre-ERT2在無Tamoxifen誘導(dǎo)的情況下,在細胞質(zhì)內(nèi)處于無活性狀態(tài);當(dāng)Tamoxifen誘導(dǎo)后,Tamoxifen的代謝產(chǎn)物4-OHT(雌激素類似物)與ERT結(jié)合,可使Cre-ERT2進核發(fā)揮Cre重組酶活性。

查看
【小鼠大學(xué)問】基因工程小鼠的命名規(guī)則

常見的基因工程小鼠可以分為兩種命名方式,包括基因定點修飾的小鼠命名,比如:敲除、敲入、點突變等等,和隨機轉(zhuǎn)基因的小鼠命名。

查看