hEPCAM

品系全名

C57BL/6Smoc-EpCAMtm1(hEpCAM)Smoc

目錄號(hào)

NM-HU-210032

品系狀態(tài)

胚胎凍存

導(dǎo)出PDF

基因信息

基因名
Epcam

基因曾用名

EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1

NCBI ID

MGI ID

Ensembl ID

Pubmed

人類同源基因

EPCAM

品系描述

利用ES細(xì)胞打靶方式，將小鼠Epcam基因全部或者部分替換為人源EPCAM。從而表達(dá)人或人鼠嵌合EPCAM蛋白，取代小鼠內(nèi)源Epcam蛋白的表達(dá)。
應(yīng)用領(lǐng)域：免疫治療；藥物篩選

驗(yàn)證數(shù)據(jù)

1.png

Fig1. Analysis of mouse EPCAM and human EPCAM protein expression in the large intestine by IHC.?Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse? expresses human EPCAM in the crypts significantly.

2.pngFig2. Analysis of mouse EPCAM and human EPCAM protein expression in the small intestine by IHC.?Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse? expresses human EPCAM in the crypts significantly.

image.png

Fig3. Detection of human EpCAM expression in WT C57 and homozygous hEpCAM mice by IHC.?Small intestine were collected from wild-type mice (+/+) and homozygous hEpCAM mice (HO/HO), and analyzed by IHC with anti-human EpCAM antibody.?

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